Added 20 μl of 10% SDS (w/v) and 20 μl of proteinase K into the tube and incubated at 55°C for 30 minutes. The lysate was cooled down and extracted once with equal volume of phenol: chloroform solution (v/v, 1:1) at 10,000rpm for 5 minutes
Added 20 μl of 10% SDS (w/v) and 20 μl of proteinase K into the tube and incubated at 55°C for 30 minutes. The lysate wascooled down and extracted once with equal volume of phenol: chloroform solution (v/v, 1:1) at 10,000rpm for 5 minutes