Next, to precisely determine the anti-HIV-1 potency
of the CRISPR/Cas9 system in the early phase of
infection, we infected T cells dually transduced with
HIV-1-specific gRNA and Cas9 with NL-GFP, which
is a VSV-G-pseudotyped, GFP-expressing HIV-1, or
NL-GFP mut, with silent alternations in the gRNAtargeting
regions of the NL-GFP virus.