4. Determination of total chlorophyll content
The extracts were diluted in 80% acetone and the absorbance of the extracts was measured
at 663 and 645 nm using UV-spectrophotometer (V-530, Jasco, Japan). The experiments were carried
out in the dark condition in order to reduce the photo destruction of the pigments. The following
equations were used for computing the quantities of pigments (mg Chl L-1):