Abstract The chemical composition of essential oils of Stevia Rebaudiana Bertoni leaves grown in
Bangladesh Sugarcane Research Institute, Pabna, Bangladesh and obtained by two different extraction
methods (hydro distillation and steam distillation) were determined by gas chromatography–
mass spectroscopy analyses (GC–MS). One hundred and twenty three peaks were obtained from
hydro distillation where 62 compounds were identified as major compounds such as a-cadinol
(2.98%), caryophyllene oxide (1.23%), ()-spathulenol (2.21%) and b-guaiene (0.32%), and 50
peaks were obtained from the steam distillation where all the major compounds were present. After
approximately 115 days the latter again ran the samples where only 4 peaks were identified of which
none of these were constituents of essential oil in Stevia Rebaudiana Bertoni. The fatty acid analysis
by GLC showed palmitic