2. Materials and methods
2.1. Experimental animalBlack tiger shrimp (P. monodon) weighing ∼20 g was procuredfrom a shrimp farm in Kundapur in Southwest coast of India, Kar-nataka. They were transported to the wet lab under aeration andacclimatized for two weeks in tanks containing sea water of 30 pptsalinity under continuous aeration.