chemicalsDried powdered marigold flower sample was provided by PTTGlobal Chemical Public Company Limited (Rayong, Thailand). Hex-ane (purity > 99.5%) used for sample preparation step was suppliedby Sigma–Aldrich. Ethanol (95% purity) and potassium hydrox-ide (KOH, purity > 99%) were purchased from Merck, USA. Diethylether (purity > 99%), ethyl acetate (purity > 99%) and sodium sulfate(Na2SO4, purity > 99%) were supplied by Merck Ltd., Thailand. Liq-uid CO2was supplied by Uchimura Sanso Co. Ltd. (Osaka, Japan)with a purity of 99.97%. Hexane (purity > 99.5%) and ethyl acetate(purity > 99%) used for SAS precipitation were supplied by WakoPure Chemical Industries Inc. (Tokyo, Japan). Lutein standards(purity > 90%) were purchased from Sigma–Aldrich, Germany