1. Introduction
Microorganisms are known to play a vital role
in technology for the production of intracellular and
extracellular enzymes on an industrial scale [1,2]. For
maximum yield, selected organisms are grown in fermenters under optimum conditions and can be further
used to make products such as cheese, bread, wine and
beer [4–6]. Most reactions inside living cells require
enzymes, which act as catalysts and are essential for life
[3,7,8].
Bacillus species are the main producers of extracellular proteases, and industrial sectors frequently use
Bacillus subtilis for the production of various enzymes.
Proteases are the main enzymes produced from microbial sources, of which only few are recommended as
commercial producers. B. subtilis is found mainly in