Probiotics application
Commercial probiotics (water, soil and gut probiotics) comprising different microbes in combination with mutagen (immuno-stimulant) (Table 1) were applied in treatment ponds using doses recommended by the manufacturer (CP Aqua, Chennai, India) while control ponds were free from probiotics application. Before and 2 days after stocking, pH fixer (mixed with freshwater and sugar) was applied (Table 2). Super biotic (mixed with freshwater and sugar) and super PS (mixed with sand) were broadcasted in the morning hours with water agitation by aerators (Tables 2 and 3). A mixture of super biotic, zymetin (gut probiotics) and mutagen (immune enhancer) were bound with sugar and coated with feed and applied twice daily (Table 4) (Saundarapandian et al. 2010).