This was carried out to determine whether any observed
inhibitory activity was due to production of antimicrobial peptides
such as bacteriocins. For each LAB isolate, CFS were separately
treated with protease (P5147, Sigma) and proteinase K (P2308,
Sigma) at a final concentration of 1 mg/ml in the supernatants.
Supernatants were incubated with the enzymes at 37 C for 2 h and