3. Results and discussion
Bacterial isolates are preferred to use at commercial level due
to short fermentation time and selective product formation but
free enzyme always faces problem due to difficult recovery from
reaction mixture which also decreases the product purity.
Immobilization is a supportive technique which not only permits
easy recovery of product and enzyme from reaction mixture but
also improves the thermal and operational stability of an enzyme.
Effect of sodium alginate concentration on entrapment of maltase
was studied different concentrations of sodium alginate. It was
observed that the percent immobilization yield of maltase was
found maximum at 4.0% sodium alginate (Fig. 1A) and as the
concentration of sodium alginate increased from maximum level,
immobilization efficiency decreased which might be due to the
formation of beads having small pore size that causes hindrance to
enter the substrate in calcium alginate beads and to react with
immobilized enzyme. However, low concentration of sodium
alginate resulted in the formation of soft and fragile beads with