Following the design, the powdered drug was passed through
the sieve (Sieve no. 10, 20 and 40) and then subjected to extraction
in Soxhlet apparatus using ethanol-water blend at different ratios
(85:15, 92.7:7.5 and 100:0) for a particular time (12 h, 18 h and
24 h) at 70 °C. The solvent was removed by concentrating the
extract in a rotary evaporator (IKA) at 40 °C under reduced pressure.
The residue left behind was reddish brown in colour and was
stored in a desiccator.