It was established by applying univariate linear regression in the range of 3.93–30g/mL. The linear equation obtained was CL emission = 171.26×[Gentamicin]−25.91 with a determination
coefficient (R2) of 99.4% and a residual standard deviation of 69.8 CL unit×ml×mg−1. The on-line linearity (97.8%) and analytical resolution (1.01 CL unit×ml×g−1) were estimated
using the calibration dataset [29]. The detection and
quantification limits according to IUPAC recommendations [36]
were 1.18 and 3.93 gml−1.