G. xylinus bacteria were cultured in
Hestrin-Schramm (HeS) medium composed of bacto-pepton e
0.5 w/v% (Oxoid, UK), glucose e 2 w/v%, yeast extract e 0.5 w/v%,
citric acid e 0.115 w/v%, Na2HPO4 e 0.27 w/v%, MgSO4$7H2O e
0.05 w/v% and ethanol e 1 v/v% (Poch, Poland) added after sterilization
of the base