The SGSautoSNP pipeline is implemented in Python 2.7 and runs from the command line on any operating system where Python is available. Most of the SGSautoSNP scripts are multithreaded to improve performance with large genomes. Other requirements are pysam , a Python module for SAM/BAM formats; Biopython ; SAMtools version and soap2sam.pl to covert SOAP results to SAM format.