Methods: The potential of cell-free culture supernatants (CFS) derived from broth cultures of selected local LAB and
yeast isolates, some of which were subjected to various treatments, were tested for inhibition of L. monocytogenes,
Salmonella spp. and S. aureus in in vitro culture by incorporating various proportions of the CFSs into the growth
medium concurrently with inoculation (co-cultures) or following limited proliferation after inoculation of the pathogens
(delayed cultures). The effects of the CFSs on various growth parameters were assessed.