To 40 ml of sample volume 10 ml of reaction buffer, containing
0.1 M sodium phosphate and 0.1 M NaCl, were added, to reach a
total volume of 50 ml, and incubated protected from light for 30 min
at 37 C. Fluorescence was measured at an excitationwavelength of
485 nmand an emissionwavelength of 535 nm (Tecan infinite 200).
Experiments were carried out in two independent series and
measured in triplicates.