After the incubation period, colonies were counted and yeast
representative colonies were isolated and sub-cultured onto YM
agar (yeast extract 3.0 g/l, malt extract 3.0 g/l, peptone, 5.0 g/l,
dextrose 10.0 g/l and agar 20 g/l) (Scharlau). Yeast colonies of all
morphotypes were selected according to their macro and micro
morphology, isolated in proportion to their frequencies and subcultured
for subsequent identification. The isolated strains were
preserved at 80 ± 5 C using glycerol (20% v/v) as cryoprotectant
agent.
After the incubation period, colonies were counted and yeastrepresentative colonies were isolated and sub-cultured onto YMagar (yeast extract 3.0 g/l, malt extract 3.0 g/l, peptone, 5.0 g/l,dextrose 10.0 g/l and agar 20 g/l) (Scharlau). Yeast colonies of allmorphotypes were selected according to their macro and micromorphology, isolated in proportion to their frequencies and subculturedfor subsequent identification. The isolated strains werepreserved at 80 ± 5 C using glycerol (20% v/v) as cryoprotectantagent.
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