Directions
Suspend 29 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at
15 lbs pressure (121°C) for 15 minutes. Just before use add 0.5 ml filter sterilized solution, prepared by mixing equal volumes
of 4% w/v solution of sodium sulphite and 7% w/v ferric citrate, to 25 ml of single strength medium or 0.4 ml and 2 ml to 10
ml and 50 ml of double strength medium respectively. Mix well.