Mitotic and meiotic metaphases were obtained from cell suspensions of intestinal epithelium and testes
respectively of animals previously treated with 2 % colchicine for 5 h, according to King & Rofe (1976) and
Schmid (1978) with few modifications. Chromosomes were stained with 10% Giemsa or submitted to the
(Ag-NOR) silver staining (Howell & Black 1980) and C-banding (Sumner 1972) techniques, with a
modification in the pre-treatment in which the samples were pre-treated with 50% acetic acid, according to
Siqueira et al. (2008). The samples were examined under a photomicroscope Olympus BX60, and images