These measurements were pooled.The shell weight/area also recorded, and shell ash was determined after drying at room temperature for 3 days.
Yolk cholesterol, blood plasma cholesterol and Low Density Lipoprotein (LDL) were determined during the last week of the trial. Yolk cholesterol was extracted by the method of Folch et al. (1956) as modified by Washburn and Nix (1974) from 2 eggs of each replicate.