The level of RT-PCR product for each growth factor is corrected according to the level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and the results are expressed as the fold-induction values. (C) Real-time PCR analysis of VEGF mRNA alternative spliced form. Real-time PCR for VEGF 121 and VEGF 165 was performed as in Section 2. The data are corrected according to the level of GAPDH and shown as the mean values _ S.E. of triplicate measurements (*P < 0.05 vs. control).