Methyl orange (MO), also
known as C.I. Acid Orange 52, is a model of anionic azo dyes, which
is an intensely colored compound and is used in dyeing and printing
textiles. Thiazine dyes are a group of basic dyes whose molecules
contain the thiazine heterocyclic. Among the thiazine dyes,
methylene blue (MB) is of the greatest industrial significance,
and is employed as a dye for temporary hair colorants, cotton,
wool, leather, and paper. MB can cause eye burn in human and
aquatic animals. It can also cause irritation to the stomach with