The contents of HTRF kit were reconstituted as per the
instructions given. In brief, the 5 kinase buffer was diluted to
1 buffer containing 5 mM MgCl2 and 1 mM DTT. STK Substrate
2 was reconstituted in distilled water to obtain a 500 mM stock
solution. Streptavidin (SA)—XL665 was reconstituted in distilled
water to obtain a 16.67 mM stock solution. The detection buffer
was reconstituted in distilled water and, used for the preparation
of STK Antibody–Cryptate and working solution of SA-XL665.
After reconstitution, the reagents were aliquoted and stored at
20 1C until further use.