choice of explants material (George et al., 2008). In this
research, we used leaf explants. On the other hand, Techato
et al. (2006) used leaves, node and internodes of
three genotypes of Anthurium. They cultured explants on
MS medium, modified MS, woody plant medium (WPM)
and Nitsch and Nitsch medium (NN) and obtained the
highest callus formation from leaf explants in MS and
modified MS. Moreover, Nhut et al. (2006) studied the
effect of ten different Anthurium genotypes and reported
different responses from their genotype. Our research
also show that the application of 2,4-D with BAP could be
positive for the propagation of A. andreanum cv. Terra.
Cimen and Ozge (2009) used 1 mg/L IBA and 0.04%
active charcoals to initiate roots. In addition, Joseph et al.
(2003) transferred shoots regenerated from A.
andreanum Hort. onto half strength MS medium
supplemented with 0.54 µM of naphthaleneacetic acid
(NAA) for rooting. The rooting process occurred on
medium without plant growth regulators. Plantlets potted
on mixture of coco pit and perlit were succesfully
acclimated.
This paper establishes a rapid method for
micropropagation of A. andreanum cv. Terra. We
concluded that in addition to mineral salt of mediums and
plant growth regulators, age and genotype of explants
are very important in the success of plant tissue culture