Leclere et al (1994) observed that, shoots produced
microtubers rapidly and in higher number
compared to nodal cutting. Myeong et al (1990)
studied the influence of several factors affecting in
vitrotuberization of shoot nodes in potato. It
reveals that, more microtubers were produced on
the nodes taken from middle and basal part of shoot
than on those from upper part. Kanwal et al. (2006)
revealed that node as an explant showed better
result on microtuber production. The present find-
Fig 4.Different types of microtuber production
under different treatments.
ing is similar with the reports of the previous
workers.
In vitrotuberization was studied on MS medium
supplemented with different concentrations of KIN
in the potato cultivar Diamant. Sprout, shoot apex
and nodal cutting were used as explant on different
concentration of sucrose for microtuber production.
It concluded that, MS medium supplemented with
6% sucrose and 4 mg/L KIN response well for all
the parameter studied. Dark condition took
minimum time for tuberization than light condition.
Maximum average weight of microtuber (397 mg)
was obtained from the treatment MS + 6% Sucrose
+ 4 mg/L KIN.