3.5. Influence of the initial sucrose concentration
Due to the low levels of residual TRS at the end of fermentation during the previous study, higher initial sugar levels were tested to avoid the possibility of limiting the generation of higher concentrations of alcohol. Thus, strains A2, A10 and A11 were evaluated by triplicate during 120 h in YPS fermentation medium containing 250, 300 and 350 g/L sucrose, using 0.5 g/L inoculum and 30 C. The profiles of ethanol production (see Fig. 6) using 250 and 300 g/L of initial sucrose were very similar using the strain A2, with a maximum final ethanol concentration of 11.61% at 120 h.