In this study, isolated strains were used to evaluate the synergistic effects of bacterial mixtures on bioremediation efficiency. At the end of the experimental period, the bacterial mixtures were found to be the most effective bacterial method, in comparison to individual culture methods. Compared with single strain cultures, the bacterial mixtures showed higher growth rate, urease activity, and resistance to heavy metals. Also, we demonstrated that the bacterial mixtures exhibited a considerably higher heavy metal bioremediation capacity than individual cultures, which might due to higher bacterial cell density at high levels of heavy metals. According to the column test results, the highest bioremediation efficiencies were noted for Pb, while the lowest removal efficiencies were detected for Cd. Bioremediation with the bacterial mixtures of isolated strains is aneffectivemethod forheavymetal removalfrom contaminated environments. This may prove to be a good strategy for developing an effective, efficient, and economic method of heavy metal bioremediation, and the introduction of these indigenous bacteria could be used for contaminated soil bioremediation without disturbing the target environment