amplified fragment length polymorphism (AFLP)
kit containing 64 primer pairs was obtained from Gibco-BRL
(Bethesda, MD, USA) and the analysis was performed following the
standard protocol (Vos et al. 1995). First, BSA was performed for
resistant and susceptible pools, taking five plants from each pool, and
then co-segregating markers were analysed for 100 individual plants.
The co-segregating AFLP markers were isolated from the denaturing
polyacrylamide gel and cloned according to Cho et al. (1996).