The Taguchi experimental design was applied to improve the diesel removal from contaminated soil and predict the optimal operating conditions using SPB1 lipopeptide biosurfactant solution. A preliminary study showed that washing potential increased with the enhancement of biosurfactant concentration. Also, results showed a tolerance to acid and alkaline pH and salinity. Better activities registered for pH values ranging from 5 to 10 and a loss between 58 and 38 % was unregistered for pH values from 2 to 4. For salinity, maximum activity was recorded when adding 5 to 15 % NaCl. An optimization study permitted an enhancement of washing capacity of about two times and an adjustment of operating conditions. In fact, a maximal removal potential of about 87 % was obtained when using 12 ml of 0.1 % biosurfactant solution at an agitation speed of 200 rpm at 30 °C and for 24 h. The reported value was comparable to chemical emulsifiers. This study revealed the adequacy of Taguchi design in promoting mobilization efficiency and demonstrated that the produced emulsifier showed great potential for use in bioremediation processes to enhance the solubility of hydrophobic compounds.