This study demonstrates the high resolving power of aqueous
two-phase extraction for separating closely related enzymes.
The technique provided a convenient means of obtaining pure
papain that was free of any contaminating protease activities. It
also allowed the direct use of papaya latex without removal of
insoluble material, which makes for a faster and simpler process
compared to other purification procedures that are limited
by the necessity to remove insoluble material from latex. High