Rice is a staple food for 90% of the world. Genetic characterization of natural resources is an essential
step to understand genetic resources. In the present study, commonly using 25 Indian rice genotypes
were collected / procured from four different states of India. Genetic variation was assessed using
isozyme and RAPD (random amplified polymorphic DNA) markers. A total of 25 isozyme putative loci
and 273 RAPD bands were generated with 64 and 96% polymorphism respectively were obtained. The
genetic similarity coefficient of isozyme study calculated from Jaccard’s similarity coefficient ranged
from 0.702 to 0.95 where as the genetic similarity coefficient of RAPD study calculated from Jaccard’s
similarity coefficient ranged from 0.328 to 0.806. Like the previous study the present indicates that
isozyme and RAPD markers are reliable, efficient and effective techniques to determine the genetic
variation in rice.