(i) A red colour produced in the lower chloroform layer when 2 ml of
organic extract was dissolved in 2 ml of chloroform and 2 ml
concentrated sulphuric acid added indicates the presence of
steroids.
(ii) The development of a greenish colour when 2 ml of the organic
extract was dissolved in 2 ml of chloroform and treated with
sulphuric and acetic acids indicates the presence of steroids.