Cow and camel milk proteins before and after heat treatment at 80 C for 60 min were identified using LC/
MS and LC–MS/MS following monodimensional electrophoresis. The database used for the identification
of camel and cow proteins was set from http://www.uniprot.org/. The obtained results showed that, after
heating, camel milk at 80 C for 60 min, camel a-lactalbumin (a-la) and peptidoglycan recognition
protein (PGRP) were not detected while camel serum albumin (CSA) was significantly diminished.
When heating cow milk at 80 C for 60 min, a-lactalbumin (a-la) and b-lactoglobulin (b-lg) were not significantly
detected. Moreover, 19 protein bands from SDS-PAGE were analyzed and a total of 45 different
proteins were identified by LC–MS/MS. Casein fractions were kept intact under a heat treatment of 80 C
during 60 min of both camel and cow milks. Camel and bovine whey proteins were affected by a heat
treatment of 80 C for 60 min.