Soil samples were collected from different localities at El-Madina El-Monawara city (Qurban El Nazel – Eastern Hora – Ohod mountain) Kingdom Saudi Arabia, and were kept at 25 °C. Human saliva samples were taken from healthy persons. One gram of soil, 1 ml of saliva and 1 ml of mixture (1 g soil + 1 ml of saliva) were inoculated to the nutrient Browth medium for bacterial growth and Sabourand Dextrose liquid medium for fungal growth, incubated for 10 days. Thereafter, the culture media were filtered using sterilized bacterial filters (NALGENE 0.45 Mm) produced by Nalge Nunc International. Isolation of different bacterial and fungal isolates was carried out from the mixture and purified. The bacterial isolates were identified according to Bergey’s manual of systematic bacteriology (Krieg and Holt, 1984 and Sneath et al., 1986). The fungal isolates were identified in Botany Department, Faculty of Science, Cairo University, Egypt.