To conduct the experiments on insecticidal action, A. aegypti
mosquitoes Rockefeller colony were kept in breeding cages
(40×40×40 cm) in an insectary at 25±5 °C, humidity of 70±
5 %, and photoperiod (12:12). Female A. aegypti were permanently offered a sugary solution of honey at 10 %, and
when larvae were needed for the experiments, they were fed
by inserting the forearm of one of the researchers for 15 minu
with prior knowledge of the ethics committee as per CEINCI UIS, Minutes No. 3 2013. Once the females were fed, they
rested in the same cage allowing them to carry out oviposition
in a container kept inside the cage lined internally with filter
paper, which served as an oviposition substrate.