The determination of γ-oryzanol was performed according to the method reported by [8] and [9] with some
modifications. A reversed phase high performance liquid chromatography (RP-HPLC) was applied using
HPLC system (model L-6200A) consisted UV–VIS photodiode array detector (Shimadzu, Japan). Separations
were conducted with a Phenomenex C18-reversed phase column (4.6×250 mm and 4 μm) (Phenomenex, USA).