Mouth of polypropylene bags were opened and the bags cut with a knife vertically from an upper point
downward and removed carefully, when whitish mycelial growth (colonization) had spread to both lower and upper
sides from the inoculated zone. Three times watering a day was done on the substrate before first harvest, one time
during second flush. The day to colonization, primordial initiation and harvest were recorded. Harvesting was done
by hand holding the stripes at the base and twisting lightly. Yield was determined by weighing and counting of fruit
bodies. BE was calculated as fresh weight of harvested mushrooms (g)/ dry weight of substrate x 100. Size of
mushrooms was calculated by total weight of fresh mushroom harvested / total number of mushroom harvested.