to release the maximum amount of compounds but with
minimal degradation and/or polycondensation. Comparable
yields with 4 and 6 N HCl at 80³C were obtained.
Hydrolysis was conducted on 1 mg of the crude polysaccharide
sample with 6 N HCl in a heating block at 80³C
for 6^8 h. The samples was cooled and the acid evaporated.
The hydrolyzed polysaccharides were resuspended
in Milli-Q water and ¢ltered through a Millipore-GX nylon
membrane prior to analysis