Stool samples were tested by microscopic examination of the stool issued on three consecutive days and by blood–agar culture. Microscopic diagnosis was based on the observation of larvae in stool samples (samples were treated in a Mini Parasep SF Faecal Parasite Concentrator). For the blood–agar culture method, stool samples were placed on a blood–agar plate and incubated for 7 days. As the larvae crawl over the agar, they carry bacteria with them, creating visible tracks.