Distilled water (200 mL) was firstly heated at 97 °C, to which
the tea bags (triplicate) were immersed for 5 minutes. During
the first 30 seconds, the bags were submersed 10 times. After
2.5 minutes, the procedure was repeated, and at the end of the
5 minutes, the tea bags were carefully removed and the tea was
immediately conditioned in a glass jar. The jar was covered to
avoid water evaporation and after cooling, the volume of the
infusion was adjusted again to 200 mL. Infusions using tea
leaves were prepared similarly using 1.5 g (also in triplicate)
of each of the samples, which were removed through filtration
after 5 minutes.