Fig. 1. In vitro propagation protocol of Dendrobium ‘Zahra FR 62’. (A) Shoot tip as explant source. (B) Shoot tip explant with several initial PLBs 3.5 months after culture initiation. (C) Initial proliferation of PLBs after second subculture. (D) PLB growth and proliferation in 3-L bioreactor with 10 vvm dissolved-oxygen containing halfstrength MS medium with 150 mL/L CW. (E) Performance of PLBs grown in 3-L bioreactor with 10 g explant density and 10 vvm dissolved-oxygen. PLB browning resulted from combination treatment between 5 g explant density with 5 vvm dissolved-oxygen. (F) Initial germination of PLBs on half-strength MS medium containing 0.05 mg/L BA ± 15days after culture. (G) Germinated PLBs 3 months after culture. (H) Well-rooted shoots harvested from rooting medium 3 months after culture. (I) Acclimatized plantlets 15days after acclimatization. (J) Good growth of individual plants after repotting. (K) Well-bloomed donor plant of D. ‘Zahra FR 62’ in the third flowering stage ± 2.5 years old.