Abstract Inthisworkweanalyzedtheeff

Abstract Inthisworkweanalyzedtheeffectsofthreeculturesystemsondevelopmentalabilityofbovineembryosinvitroproduced withsexedsperm,thesurvivaltovitrification(cryologicvitrificationmethod)ofsuchblastocysts,andtheirpregnancyratesafter embryotransfertorecipients,bothasfreshandaftervitrification/warming.Finally,wemeasuredtheaccuracyofthesorting protocolbyapolymerasechainreaction-basedmethodtovalidatetheembryosexatblastocyststages.Weconfirmedanindividual effectofthebullaswellasdevelopmentratesofembryosproducedwithsortedspermlowerthanembryoswithunsortedsperm, independentoftheculturesystemused.Thecryoresistancetovitrificationofembryosproducedwithsexedspermdidnotdiffer fromthatofconventionallyproducedembryos(re-expansionratesat24and48h:74.6%vs.75.5%,and64.5%vs.68.1%for embryosproducedwithconventionalandsortedsperm,respectively;hatchingratesat48h:63.55%vs.55.5%forembryos producedwithconventionalandsortedsperm,respectively).Finally,nosignificantdifferenceswerefoundinpregnancyratesafter theembryotransferoffreshandvitrified/warmedblastocysts(52.8%vs.42.0%,respectively;P0.05).Maleandfemaleembryos producedwithsortedspermshowedthesamequalityintermsofdevelopmentalability,cryoresistance,andpregnancyratesafter transfer.Ourculturesystem,coupledwiththevitrificationinfiberplugs,providesgoodqualitysex-knownembryoswhichsurvive vitrificationatsimilarratesthanembryosproducedwithconventionalunsortedsperm;alsoitproducesgoodpregnancyratesafter transferofsexedembryosbothfreshandaftervitrificationandwarming. ©2012ElsevierInc.Allrightsreserved. Keywords:Sex-sortedsperm;Invitroproduction;Vitrification;Embryotransfer;Pregnancy

ผลลัพธ์ (ไทย) 1:

นามธรรม inthisworkweanalyzedtheeffectsofthreeculturesystemsondevelopmentalabilityofbovineembryosinvitroproduced withsexedsperm,thesurvivaltovitrification(cryologicvitrificationmethod)ofsuchblastocysts,andtheirpregnancyratesafter embryotransfertorecipients, bothasfreshandaftervitrification / warming.finally,wemeasuredtheaccuracyofthesorting protocolbyapolymerasechainreaction-basedmethodtovalidatetheembryosexatblastocyststages.weconfirmedanindividual effectofthebullaswellasdevelopmentratesofembryosproducedwithsortedspermlowerthanembryoswithunsortedsperm, independentoftheculturesystemusedthecr​​yoresistancetovitrificationofembryosproducedwithsexedspermdidnotdiffer fromthatofconventionallyproducedembryos(re-expansionratesat24and48h:74.6%vs.75.5%,and64.5%vs.68.1%for embryosproducedwithconventionalandsortedsperm,respectively;hatchingratesat48h:63.55%vs.55.5%forembryos producedwithconventionalandsortedsperm ตามลำดับ). ในที่สุดnosignificantdifferenceswerefoundinpregnancyratesafter theembryotransferoffreshandvitrified/warmedblastocysts(52.8%vs.42.0%,respectively;p0.05).maleandfemaleembryos producedwithsortedspermshowedthesamequalityintermsofdevelopmentalability,cryoresistance,andpregnancyratesafter transfer.ourculturesystem, coupledwiththevitrificationinfiberplugs,providesgoodqualitysex-knownembryoswhichsurvive vitrificationatsimilarratesthanembryosproducedwithconventionalunsortedsperm;alsoitproducesgoodpregnancyratesafter transferofsexedembryosbothfreshandaftervitrificationandwarming © 2012elsevierinc.allrightsreserved คำหลัก: เพศ sortedsperm; invitroproduction; แก้ว; embryotransfer; การตั้งครรภ์

ผลลัพธ์ (ไทย) 2:

บทคัดย่อ Inthisworkweanalyzedtheeffectsofthreeculturesystemsondevelopmentalabilityofbovineembryosinvitroproduced withsexedsperm, thesurvivaltovitrification (cryologicvitrificationmethod) ofsuchblastocysts, andtheirpregnancyratesafter embryotransfertorecipients, bothasfreshandaftervitrification/ภาวะ โลกร้อนสุดท้ายwemeasuredtheaccuracyofthesorting protocolbyapolymerasechainreaction-basedmethodtovalidatetheembryosexatblastocyststagesWeconfirmedanindividual effectofthebullaswellasdevelopmentratesofembryosproducedwithsortedspermlowerthanembryoswithunsortedsperm, independentoftheculturesystemusedThecryoresistancetovitrificationofembryosproducedwithsexedspermdidnotdiffer fromthatofconventionallyproducedembryos (re-expansionratesat24and48h:74.6%vs.75.5%,and64.5%vs.68.1%for embryosproducedwithconventionalandsortedsperm,respectively;hatchingratesat48h:63.55%vs.55.5%forembryos producedwithconventionalandsortedsperm ตามลำดับ)สุดท้ายnosignificantdifferenceswerefoundinpregnancyratesafter theembryotransferoffreshandvitrified/warmedblastocysts(52.8%vs.42.0%,respectively;P0.05)Maleandfemaleembryos producedwithsortedspermshowedthesamequalityintermsofdevelopmentalability, cryoresistance, andpregnancyratesafter โอนOurculturesystem, coupledwiththevitrificationinfiberplugsprovidesgoodqualitysex-knownembryoswhichsurvive vitrificationatsimilarratesthanembryosproducedwithconventionalunsortedsperm; alsoitproducesgoodpregnancyratesafter transferofsexedembryosbothfreshandaftervitrificationandwarming © 2012ElsevierInc.Allrightsreserved คำสำคัญ: เพศ-sortedsperm; InvitroproductionVitrificationEmbryotransferการตั้งครรภ์

ผลลัพธ์ (ไทย) 3:

นามธรรม inthisworkweanalyzedtheeffectsofthreeculturesystemsondevelopmentalabilityofbovineembryosinvitroproduced withsexedsperm , thesurvivaltovitrification ( cryologicvitrificationmethod ) ofsuchblastocysts , andtheirpregnancyratesafter embryotransfertorecipients , bothasfreshandaftervitrification /อุ่น.สุดท้าย,wemeasuredtheaccuracyofthesorting protocolbyapolymerasechainreaction - basedmethodtovalidatetheembryosexatblastocyststages . weconfirmedanindividual effectofthebullaswellasdevelopmentratesofembryosproducedwithsortedspermlowerthanembryoswithunsortedsperm , independentoftheculturesystemused .thecryoresistancetovitrificationofembryosproducedwithsexedspermdidnotdiffer fromthatofconventionallyproducedembryos (ใหม่ - expansionratesat 24 และ 48 ชั่วโมง: 74.6% เมื่อเทียบกับ 75.5% , และ 64.5% เมื่อเทียบกับ 68.1% สำหรับ embryosproducedwithconventionalandsortedsperm ,ตามลำดับ; hatchingratesat 48 ชั่วโมง: 63.55% เมื่อเทียบกับ 55.5% forembryos producedwithconventionalandsortedsperm ตามลำดับ)..สุดท้าย,theembryotransferoffreshandvitrified nosignificantdifferenceswerefoundinpregnancyratesafter / warmedblastocysts ( 52.8% เมื่อเทียบกับ– 42.0% ตามลำดับ P 0.05 ) producedwithsortedspermshowedthesamequalityintermsofdevelopmentalability maleandfemaleembryos cryoresistance andpregnancyratesafter ถ่ายโอน. ourculturesystem coupledwiththevitrificationinfiberplugsvitrificationatsimilarratesthanembryosproducedwithconventionalunsortedsperm providesgoodqualitysex - knownembryoswhichsurvive transferofsexedembryosbothfreshandaftervitrificationandwarming alsoitproducesgoodpregnancyratesafter . © 2012 elsevierinc .สงวนลิขสิทธิ์. คำหลัก:เพศ - sortedsperm invitroproduction กลายเป็นแก้ว embryotransfer การตั้ง ครรภ์