Fabian and Ju, (2011) reported that several treatment methods (physical, heat, enzymic and chemical)have been used in the extraction of protein from rice bran. The authors further reported that the use of enzyme and subcritical water treatment showed promising protein yield among all the available pretreatment methods, but the relative expensive cost of enzyme and the quality of protein extracted at relatively high temperatures for subcritical water treatment constitutes limitations to the use of the methods due to reduced protein functionality
(Fabian and Ju, 2011). Shih (2003) found that treatment of rice bran by chemical, enzyme and
heat methods prior to protein extraction could affect, often adversely, the protein functionality.