Finally, the amplified polymerase chain reaction (PCR) products
were analysed in a 1.0 (w/v) % agarose gel in 1x TAE buffer at 90 V
for 65 min; and gels were visualized by using gel documentation
system (Alpha Imager). The amplified DNA fragments were purified
and sent to 1st Base for automated DNA sequencing (1st Base
Malaysia, Sdn Bhd). The homology of the sequences were
analyzed using the BLASTN programs and compared with the
Genbank database at National Center for Biotechnology Information
(NCBI) accessible on-line at http://www.ncbi.nlm.nih.gov/
Genbank/