tThis study focused on the production of a novel multi-CLEA comprising the enzyme activities of lipaseand protease from fish viscera. A multi-CLEA is a single biocatalyst that can catalyze separate unrelatedreactions, but these reactions can be conducted in one application. Tests pertaining to the effect of variousadditives on the multi-CLEA’s activity were performed. Response Surface Methodology’s Face CenteredCentral Composite Design (FCCCD) was employed to optimize the preparation parameters of the multi-CLEA in an aqueous medium. It was found that 55% (w/v) of ammonium sulfate, 65 mM of glutaraldehyde,and 0.113 mM of bovine serum albumin were the optimum levels of additives to prepare the multi-CLEAwith the protease and lipase recovery activity of 43.82% and 99.91%, respectively. Multi-CLEAs werefound to retain an average of more than 34% of the initial activity after five consecutive batches for bothenzymes. Finally, the multi-CLEA was utilized to catalyze two reactions: improved washing process andbiodiesel production. The stain removal percentage of a commercial detergent was improved by 67.78%after adding multi-CLEA. In addition, the multi-CLEA catalyzed biodiesel production from vegetable oilwith a percentage conversion of 51.7%. Such results demonstrated that the multi-CLEA is a promisingcatalyst for biotechnological applications.