Analysis
Determination of Total phenolic content (TPC)
The samples 300 µl will be introduced into test tubes and then followed by 1.5 ml of Folic-Ciocalteu reagent (10 times dilution) and 1.2 ml of sodium carbonate (7.5% w/v).
The tubes will be allowed to stand for 30 min.
To measure the absorption at 765 nm.