, the
supernatant was eliminated, DNA pellets were washed with 500 mL
of 70% ethanol, and tubes were centrifuged at 12 000 g for 5 min.
The ethanol was then discarded and the pellets were air dried at
room temperature for several hours. Finally, the DNA was resuspended
in 100 mL of ultra-pure water and stored at 20 C until
analysis. DNA quantities were estimated by electrophoretic
migration through a 0.8% agarose gel and by using a UV spectrophotometer
(BioSpec-Nano, Shimadzu). Gels were photographed
on a UV transilluminator with a CCD camera and Gel Smart 7.3
system (Clara Vision).