These liquid culture media were distributed into 245 mL replications in 500 mL glass bottles (2 replications for each experiment A00 and B00, and 1 replication for experiment C00). Bottles
of experiments A00 and B00 were inoculated with 5 mL of the bacteria inoculum, and 5 mL of sterile 0.8% NaCl was added to the bottle of experiment C00. All bottles were closed and incubated without agitation at 25 C under anaerobic conditions for 31 days. An aliquot of 5 mL from each bottle was taken each 2–3 days. Firstly, biomass was measured by absorbance readings at 600 nm (using the synthetic wine as a blank).