3.1 Determination of Mn Fifty percent inhibition concentration (IC50) on Acanthamoeba sp.
In this study, Acanthamoeba sp. were exposed to various concentrations of Mn i.e 0, 30, 60, 90, 120, 150 and
180 ppm for 24 hours at 30qC in a 96-well plate to determine the cytotoxicity of the metal. The MTT assay was
carried out to assess the viability of Acanthamoeba sp. Fig.1 shows the graph of viability of cell against different
concentrations of Mn. Based on the graph, the IC50 value obtained was 24 ppm and Acanthamoeba sp. showed the
reduction in viability upon exposure to increasing in Mn concentration.