section of the forespore (FS in diagram) and mother cell (MC in diagram). (C) MurG-YFP distribution
in a population of sporulating cells in resuspension (JDB1689). Fluorescence images on top are an
overlay of YFP (green) with FM4-64 dye (red). Bottom is MurG-YFP signal only. Time in hours after
resuspension is indicated. (D) Graph shows the forespore to mother cell ratio of fluorescence for
MurG-YFP (black line JDB1689) and free CFP (blue line JDB2157) at different time-points
during sporulation. (E) Diagrams of MurG-YFP distribution. MurG-YFP is shown in green. (F) Diagram
showing the forespore to mother cell fluorescence ratios (FS/MC) in three different distributions of
fluorescent proteins (in green). Left, diagram shows a cytoplasmic protein with no specific
localization, FS/MC1. Middle, diagram shows a membrane bound protein with no specific
localization, FS/MC>1. Right, diagram shows a membrane bound protein, specifically localized to the
forespore, FS/MC >> 1. (For interpretation of the references to color in this figure legend, the
reader is referred to the web version of this article.)